Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections.

摘要: Germinal centres are areas of intense B lymphocyte proliferation inside primary cell follicles in spleen and lymph nodes. Rearranged V genes from single human cells, isolated histological sections two such structures by micromanipulation, were amplified sequenced. Cells the follicular mantle clonally diverse largely expressed germline genes. dominated a few large clones dispersed throughout these exhibiting intraclonal diversity ongoing somatic hypermutation. Pronounced counterselection replacement mutations seen one germinal may indicate late phase centre reaction. A polyclonal population activated cells expressing unmutated antibodies dark zone other represent initial founder cells.