Toxicity methods for CPPs.
作者： Per Lundin , Samir EL Andaloussi , Ülo Langel
DOI: 10.1007/978-1-60761-919-2_14
关键词: Pharmacology 、 In vitro cytotoxicity 、 Cytotoxicity 、 Membrane integrity 、 Viability assay 、 Toxicity 、 Chemistry 、 In vivo 、 MTT assay 、 Propidium iodide
摘要: CPPs have for numerous years been utilized as delivery vectors of various pharmaceutically interesting cargoes, both in vitro and vivo. As are gradually approaching the bedsides, investigating toxicity associated with these highly peptides becomes increasingly important thorough initial assessment cytotoxicity is a first step towards advancing vehicles to clinics. The present chapter describes protocols four assays order provide toolbox CPPs. foci lie on membrane integrity (deoxyglucose leakage propidium iodide assays) cell viability (the MTT assay), but also provides protocol assessing an parameter future clinical applications, namely hemolytic properties
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