Effect of poly-glutamate on uptake efficiency and cytotoxicity of cell penetrating peptides.
作者： Samad Mussa Farkhani , Ali Shirani , Samaneh Mohammadi , Parvin Zakeri‐Milani , Javid Shahbazi Mojarrad
DOI: 10.1049/IET-NBT.2015.0030
关键词: Polyglutamic acid 、 Cytotoxicity 、 Cell 、 Cell biology 、 A549 cell 、 Drug delivery 、 Cancer cell 、 Materials science 、 Toxicity 、 Flow cytometry
摘要: Cell penetrating peptides (CPPs) were developed as vehicles for efficient delivery of various molecules. An ideal CPP-peptide should not display any toxicity against cancer cells well healthy and efficiently enter into the cell. Because cationic nature intrinsic vector capabilities, these can cause cytotoxicity. One possible reasons CPPs is direct translocation consequently, pore formation on plasma membrane. In this study it was demonstrated that interaction poly-glutamate with CPP considerably reduced their cytotoxicity in A549 This strategy could be useful drug mediated by CPP.
sci-hub.se 参考文章(40)
Per Lundin, Samir EL Andaloussi, Ülo Langel, Toxicity methods for CPPs. Methods of Molecular Biology. ,vol. 683, pp. 195- 205 ,(2011) , 10.1007/978-1-60761-919-2_14
Thomas J. McIntosh, Sidney A. Simon, Peptide-lipid interactions Academic Press. ,(2002)
Annely Lorents, Praveen Kumar Kodavali, Nikita Oskolkov, Ülo Langel, Mattias Hällbrink, Margus Pooga, Cell-penetrating Peptides Split into Two Groups Based on Modulation of Intracellular Calcium Concentration Journal of Biological Chemistry. ,vol. 287, pp. 16880- 16889 ,(2012) , 10.1074/JBC.M111.318063
Hanna A. Rydberg, Nils Carlsson, Bengt Nordén, Membrane interaction and secondary structure of de novo designed arginine-and tryptophan peptides with dual function. Biochemical and Biophysical Research Communications. ,vol. 427, pp. 261- 265 ,(2012) , 10.1016/J.BBRC.2012.09.030
Helene L. Åmand, Carolina L. Boström, Per Lincoln, Bengt Nordén, Elin K. Esbjörner, Binding of cell-penetrating penetratin peptides to plasma membrane vesicles correlates directly with cellular uptake. Biochimica et Biophysica Acta. ,vol. 1808, pp. 1860- 1867 ,(2011) , 10.1016/J.BBAMEM.2011.03.011
Guillaume Drin, Sylvine Cottin, Emmanuelle Blanc, Anthony R. Rees, Jamal Temsamani, Studies on the Internalization Mechanism of Cationic Cell-penetrating Peptides * Journal of Biological Chemistry. ,vol. 278, pp. 31192- 31201 ,(2003) , 10.1074/JBC.M303938200
Soo Hyeon Lee, Bastien Castagner, Jean-Christophe Leroux, Is there a future for cell-penetrating peptides in oligonucleotide delivery? European Journal of Pharmaceutics and Biopharmaceutics. ,vol. 85, pp. 5- 11 ,(2013) , 10.1016/J.EJPB.2013.03.021
Marta Soler, Marta González-Bártulos, David Soriano-Castell, Xavi Ribas, Miquel Costas, Francesc Tebar, Anna Massaguer, Lidia Feliu, Marta Planas, Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties Organic and Biomolecular Chemistry. ,vol. 12, pp. 1652- 1663 ,(2014) , 10.1039/C3OB42422G
Wouter P.R. Verdurmen, Melissa Thanos, Ivo R. Ruttekolk, Erich Gulbins, Roland Brock, Cationic cell-penetrating peptides induce ceramide formation via acid sphingomyelinase: implications for uptake. Journal of Controlled Release. ,vol. 147, pp. 171- 179 ,(2010) , 10.1016/J.JCONREL.2010.06.030
Hisaaki Hirose, Toshihide Takeuchi, Hiroko Osakada, Sílvia Pujals, Sayaka Katayama, Ikuhiko Nakase, Shouhei Kobayashi, Tokuko Haraguchi, Shiroh Futaki, Transient focal membrane deformation induced by arginine-rich peptides leads to their direct penetration into cells. Molecular Therapy. ,vol. 20, pp. 984- 993 ,(2012) , 10.1038/MT.2011.313