Expression Cloning of an Interferon-inducible 17-kDa Membrane Protein Implicated in the Control of Cell Growth*
作者： Gisèle A. Deblandre , Olivier P. Marinx , Sharon S. Evans , Samira Majjaj , Oberdan Leo
关键词: Molecular biology 、 Expression cloning 、 Cell biology 、 Membrane protein 、 Retinoblastoma-like protein 1 、 HSPA9 、 Biology 、 Complementary DNA 、 Vesicle-associated membrane protein 8 、 HSPA4 、 HSPA2
摘要: Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity interferons based on (1) their pattern induction interferon-sensitive and -resistant cell lines (2) ability fraction enriched 17-kDa inhibit growth. To gain insight into nature that mediate interferons, monoclonal antibody, 13A5, was generated reacted specifically with interferon-inducible surface protein. The expression this protein by human correlated sensitivity interferons. obtain information regarding structure protein, 13A5 antibody used screen COS cells transfected cDNA library. Sequence analysis full-length clone revealed identity 9-27 cDNA, previously isolated basis its interferon inducibility differential screening. In addition, encoded gene shown be identical Leu-13 antigen. identified as 16-kDa leukocytes endothelial is component multimeric complex involved transduction homotypic adhesion signals. These results suggest novel level cellular regulation involving gene, which associates other at surface, forming relaying growth inhibitory aggregation
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