Chicken egg yolk antibodies (IgY) for detecting circulating antigens of Schistosoma japonicum.
作者： Yu-Chun Cai , Jian Guo , Shao-Hong Chen , Li-Guang Tian , Peter Steinmann
DOI: 10.1016/J.PARINT.2012.01.008
关键词: Antigen 、 Immunoassay 、 Polyclonal antibodies 、 Immunology 、 Positive reaction 、 Microbiology 、 Biology 、 Schistosoma japonicum 、 Yolk 、 Positive response 、 Antibody
摘要: Abstract Background IgY isolated from egg yolk has been widely used in immunodiagnostic tests, including tests to detect circulating antigen (soluble or SEA) of Schistosoma japonicum . Results A sandwich ELISA was established using a combination anti- S. SEA-IgY polyclonal antibodies and IgM monoclonal antibodies. To explore sensitivity specificity the ELISA, serum samples 43 patients infected with were tested. All acute cases 91.3% chronic showed positive reaction. Only 5% control sera healthy persons gave response. Cross-reactions nine other parasites rare. Conclusion The developed immunoassay is reasonably sensitive specific. It could be for field research treatment efficacy assessments.
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