Tracers for investigating pathogen fate and removal mechanisms in mesocosms
作者： Alan G. Werker , Wendy Van Loon , Raymond L. Legge
DOI: 10.1016/J.SCITOTENV.2006.12.034
关键词: Particle 、 Removal kinetics 、 Microsphere 、 Portable water purification 、 Environmental engineering 、 TRACER 、 Mesocosm 、 Fluorescence 、 Biology 、 Wastewater 、 Environmental chemistry
摘要: The purpose of the present investigation has been to develop a tracer suite that application in in-situ assessment and optimization physical biological removal elimination mechanisms pathogens within laboratory scale treatment systems. includes three pathogen indicators, namely, conserved non-viable particle (fluorescently labelled microspheres, FLM), non-conserved bioparticles, FLB), viable (Nalidixic acid resistant E. coli, NAREC). triplet principles were developed with practical experiments on planted, unplanted subsurface flow wetland mesocosms treating synthetic domestic wastewater. tracers monitor for (FLM), activity (FLB), thresholds (NAREC). FLM enumeration was simplified by calibration concentration respect acetone-extractable fluorescence. Similarly, FLB assessed bulk fluorescence using two characteristic excitation-emission wavelength pairs: 494/519 220/319 nm. NAREC results indicated first order kinetics may only proceed down limiting threshold concentrations.
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