Improvement of hydrogen production with thermophilic mixed culture from rice spent wash of distillery industry

摘要: Abstract Biohydrogen production processes were investigated using thermophilic bacterial consortia enriched from sludge of the anaerobic digester. A multiple parameter optimization viz. temperature, pH and substrate concentration was performed for maximization hydrogen production. Heat shock pre-treatment followed by BES (2-bromo ethane sulfonate) treatment done enrichment producing bacteria. Box–Behnken design response surface methodology adopted to investigate mutual interaction among process parameters. Experimental parameters (60 °C, 6.5 10 g/L) gave maximum yield 3985 mL/L 2.7 mol/mol glucose respectively in batch system which is higher than reported value on UASB. These experimental found concurrent with values obtained theoretical model i.e. 58.4 °C, 6.6, 10.8 g/L 2.71 mol/mol glucose. At optimized conditions, rate ( R m ) 850 mL/h, gas potential P 4551 mL/L lag time λ 1.98 h determined modified Gompertz equation. Using optimum rice spent wash conducted 464 mL/g carbohydrate 168 mL/L h obtained. PCR-DGGE profile showed that mixed culture predominated species closely affiliated Thermoanaerobacterium sp.