MALDI-FTICR MS Imaging as a Powerful Tool to Identify Paenibacillus Antibiotics Involved in the Inhibition of Plant Pathogens

摘要: Nowadays, microorganisms are more and often used as biocontrol agents for crop protection against diseases. Among them, bacteria of Bacillus Paenibacillus genders already commercial agents. Their mode action is supposed to be related their production antibiotics, such cyclic lipopeptides, which exhibit great antimicrobial activities. We chose work with a polymyxa strain (Pp56) very resistant various microorganisms. The were grown simultaneously Fusarium oxysporum we applied matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) mass spectrometry identify the antibiotics compounds present in fungus growth inhibition area. We, therefore, identified fusaricidins A, B, C numerous members LI-F family. MALDI-FTICR imaging was then follow diffusion lipopeptides involved inhibitory activity over time. analyzed molecular content area at different Pp56 incubation durations concluded that some fusaricidin B mixture LI-F05b/06b/08a mainly defense mechanism Pp56. Our study confirms MALDI may powerful tool quickly determine species an antagonism another microorganism, avoiding time-consuming steps extraction, purification, tests, still commonly microbiology.