Imaging secondary metabolism of Streptomyces sp. Mg1 during cellular lysis and colony degradation of competing Bacillus subtilis

摘要: Soil streptomycetes are saprotrophic bacteria that secrete numerous secondary metabolites and enzymes for extracellular functions. Many produce antibiotics thought to protect vegetative mycelia from competing organisms. Here we report an organism isolated soil, Streptomyces sp. Mg1, actively degrades colonies causes cellular lysis of Bacillus subtilis when the organisms cultured together. We predicted inhibition degradation B. in this competition depends upon a combination secreted factors, including small molecule enzymes. To begin unravel complex competitive phenomenon, use MALDI imaging mass spectrometry strategy map positions by both In report, show Mg1 produces macrolide antibiotic chalcomycin A, which contributes growth with other, as yet unidentified factors. suggest efforts understand cooperative interactions between bacterial species benefit assays pair living probe complexity metabolic exchanges them.