Analysis of tumour necrosis factor alpha-specific, lactose-specific and mistletoe lectin-specific binding sites in human lung carcinomas by labelled ligands.

摘要: Receptor sites can be visualized by labelled ligands as an alternative to receptor-specific antibodies, substantiated for two different receptor classes. Recombinant tumour necrosis factor alpha (TNF) was biotinylated via amino-groups and the resultant probe applied formalin-fixed, paraffin-embedded tissue sections of 94 primary bronchial carcinomas normal peripheral lung parenchyma. In addition, monoclonal antibodies specific neuron-specific enolase (NSE) TNF itself were used. The beta-galactoside-specific mistletoe lectin, which exhibits dose-dependent immunomodulatory toxic potency, probes that specifically detect certain types sugar receptors employed illustrate further feasibility using localisation. tumours comprised 62 small cell carcinomas, 10 epidermoid 11 adenocarcinomas large anaplastic carcinomas. Expression TNF-binding found in 39 13 non-small Binding capacity TNF-specific antibody seen similar proportions None parenchymas revealed significant staining. capacities lectin all nearly cases adenocarcinoma (10/11). A correlation between expression NSE binding detected. Endogenous lectins, lactose or beta-GalNAc, displayed one half carcinoma (44% 45% respectively) about 25% cases.