Individualized cancer therapy
作者： David M. Shanahan , Donald Rao , Phillip B. Maples , Neil Senzer , I John J. Nemunaitis
DOI:
关键词: RNA 、 Molecular biology 、 Cancer therapy 、 Gene 、 Cancer research 、 Biology 、 Gene chip analysis 、 RNA interference 、 Delivery vehicle 、 Expression cassette 、 Cancer
摘要: In certain preferred embodiments, the invention provides methods for treating cancer, which comprise (a) obtaining a specimen of cancer tissue from patient; (b) normal in proximity such (c) extracting total protein and RNA tissue; (d) expression profile using 2D DIGE mass spectrometry; (e) identifying proteins that are expressed at significantly different levels than (f) gene microarray technology comparing results thereof to profile; (g) prioritizing over-expressed by assessing connectivity other cancer-related or stimulatory proteins; (h) designing an appropriate interference cassette to, directly indirectly, modulate genes encoding prioritized (i) incorporating said into delivery vehicle; (j) providing patient with effective amount vehicle modify (i.e., production) proteins.
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