The meiotic spindle of the Drosophila oocyte: the role of Centrosomin and the central aster

摘要: We provide here the first evidence that a distinct midzone is present in Drosophila melanogaster female meiosis I spindle. This region has ability to bind Pavarotti kinesin-like (PAV-KLP) and Abnormal spindle (Asp) proteins, indicating correct organization of central microtubules. also identified core component centrosomal protein centrosomin (CNN) at an unexpected site within anaphase spindle, role for CNN during biogenesis meiotic apparatus. However, there are no apparent defects cnn oocytes, whereas occur later when aster forms. The primary mutant phenotype oocytes failure form developed microtubule organizing center (MTOC), although twin II spindles usually do form. Thus MTOC may not be essential formation inner poles spindles, as generally proposed, but it might involved maintaining their proper spacing. discuss proposal that, presence MTOC, chromatin-driven mechanism assembly like described control morphogenesis spindles.