Quantification of Viral RNA and DNA Positive Cells in Tissues From Simian Immunodeficiency Virus/Simian Human Immunodeficiency Virus Infected Controller and Progressor Rhesus Macaques.

摘要: Eradication of human immunodeficiency virus 1 (HIV-1) from an infected individual cannot be achieved using current antiretroviral therapy (ART) regimens. Viral reservoirs established in early infection remain unaffected by ART and are able to replenish systemic upon treatment interruption. Simian (SIV) macaque models useful for studying HIV pathogenesis, treatments, persistent viral reservoirs. Here, we used the SIV model examine quantify RNA DNA positive cells tissues macaques that control replication (controllers) those have persistently high plasma viremia (progressors). A correlation was detected between tissue RNA+ load both mesenteric lymph node (LN) spleen. Similarly, a also observed DNA+ ileum jejunum. Controllers had lower frequency several compared progressors. However, were prevalent LN, inguinal colon, midbrain, bone marrow controller Organized lymphoid LNs, spleen, intestine found as major virus. brain thymus with SIV-encephalitis. Both T macrophages shown tissues, indicating vaccines should specifically designed protect these organized tissues. These results indicate target secondary organs reduce productively latently cells.