Recently Infiltrating MAC387+ Monocytes/Macrophages: A Third Macrophage Population Involved in SIV and HIV Encephalitic Lesion Formation

摘要: Monocytes/macrophages are critical components of HIV and SIV encephalitic lesions. We used in vivo BrdU labeling markers specific to stages macrophage differentiation or inflammation define heterogeneity better the role populations lesion formation productive infection. Lesions were heterogeneously composed resident macrophages (CD68 + HAM56 ), perivascular (CD163 CD68 MAC387 − recently infiltrated CD163 monocytes/macrophages. At 24 48 hours after inoculation, 30% monocytes/macrophages , consistent with their being infiltrated. In cuffs low-level replication, outnumbered macrophages. Conversely, lesions numerous SIV-p28 multinucleated giant cells had fewer The not productively infected nor did they express detectable CCR2, unlike Overall, we found that proportion tends be higher than brain animals mild encephalitis; ratio was reversed more severe encephalitis. These results suggest development encephalitis is an active ongoing process involves recruitment accumulation of: i) nonproductively present (potentially M1-like macrophages), ii) (consistent M2-like iii) latter two cellular reservoirs for