An examination of the metabolic processes underpinning critical swimming in Atlantic cod (Gadus morhua L.) using in vivo 31P-NMR spectroscopy.

摘要: Traditionally, critical swimming speed has been defined as the when a fish can no longer propel itself forward, and is exhausted. To gain better understanding of metabolic processes at work during U(crit) swim test, that lead to fatigue, we developed method using in vivo (31)P-NMR spectroscopy combination with Brett-type tunnel. Our data showed transition point reached change from steady state aerobic metabolism non-steady anaerobic metabolism, indicated by significant increase inorganic phosphate levels 0.3+/-0.3 9.5+/-3.4 mol g(-1), drop intracellular pH 7.48+/-0.03 6.81+/-0.05 muscle. This coincides gait subcarangiform kick-and-glide bursts. As number kicks increased, so too did Pi concentration, pH(i) dropped. Both changes were maximal U(crit). A Gibbs free energy ATP hydrolysis -55.6+/-1.4 -49.8+/-0.7 kJ mol(-1) argued have involved fatigue. confirms earlier findings traditional definition U(crit), unlike other points are typically marked complete exhaustion both resources.