Adenovirus early region 1B 58,000-dalton tumor antigen is physically associated with an early region 4 25,000-dalton protein in productively infected cells.

摘要: Abstract In soluble protein extracts obtained from adenovirus productively infected cells, monoclonal antibodies directed against the early region 1B 58,000-dalton (E1B-58K) immunoprecipitated, in addition to this protein, a polypeptide of 25,000 molecular weight. An analysis tryptic peptides derived 25K demonstrated that it was unrelated E1B-58K protein. The peptide maps produced 5 (Ad5)-infected HeLa cells and BHK were identical, whereas Ad3-infected different viral origin confirmed by an amino acid sequence determination five methionine residues two Ad2 positions these compared with nucleotide uniquely mapped gene for 4, subregion 6 genome. A mutant Ad5 (Ad5 dl342) which failed produce detectable levels In mutant, detect associated 293 dl342, contain encoded integrated genome, E4-25K Extracts labeled dl342-infected (E1B-58K-) mixed vitro unlabeled wild type-infected or (E1B-58K+). When incubated antibody, coimmunoprecipitated. uninfected (both E1B-58K-) mixed, antibody immunoselect These data provide evidence antigen is physically cells. This same previously shown be cellular p53 adenovirus-transformed