Crocin Effects on Human Myeloma Cells Regarding Intracellular Redox State, DNA Fragmentation, and Apoptosis or Necrosis Profile

摘要: Background: Well-documented studies reported several pharmacological properties for crocin, the active compound of Crocus sativus, such as its antitumor, radical scavenging, antidepressant, and memory-enhancing effects. Objectives: We aimed to evaluate possible cytotoxic activity crocin on B lymphocytes in human myeloma (U266 cell line) after 24- 48-hour treatment. Materials Methods: For this purpose, viability was determined by colorimetric MTT assay death pattern evaluated using Annexin V-FITC/propidium iodide (PI) apoptosis detection kit. ROS (reactive oxygen species) production DNA fragmentation were assessed 2â�²,7â�²-dichlorofluorescein diacetate (DCFH-DA) kit PI staining, respectively. Results: The highest concentration significantly decreased 48 hours However, had no effect expression level HSP (Heat shock protein). Additionally, administration caused a mild decline increase population fragmented cells well apoptosis. Conclusions: In our study, prominent seen; therefore, order have better perspective against cancerous lines, further are highly recommended. © 2014, School Pharmacy, Ahvaz Jundishapur University Medical Sciences.