Automated polymerase chain reaction product sample preparation for capillary electrophoresis analysis

摘要: The analysis of crude polymerase chain reaction (PCR) products by capillary electrophoresis (CE) is often compromised due to the presence a high concentration salt. Salt interferes with electrokinetic injection and induces localized heating within column; hence, PCR must be desalted or cleaned-up prior CE analysis. A variety commercial clean-up systems are available that have been traditionally used prepare for cloning, sequencing digestion restriction enzymes. These were tested their effectiveness in preparing evaluated based on resolution, salt removal, DNA recovery, processing time cost. One particularly effective system, membrane dialysis, was automated using robotic workstation.