High-speed and high-accuracy DNA sequencing by capillary gel electrophoresis in a simple, low cost instrument Two-color peak-height encoded sequencing at 40°C
作者： Hui Lu , Edgar Arriaga , Yong Chen Da , Norman J. Dovichi
DOI: 10.1016/0021-9673(94)85148-4
关键词:
摘要: Abstract A low-cost DNA sequencer was constructed based on a single helium-neon laser. The two-color peak-height encoded sequencing protocol, the use of T7 polymerase in manganese buffer, used to generate samples. Two termination reactions were performed. In first, TAMRA (applied Biosystems)-labeled primer extended presence ddATP and ddCTP. amounts dideoxynucleotides adjusted produce 3:1 peak height ratio. Similarly, ROX (Applied ddGTP ddTTP; pooled fragments separated 4% T LongRanger gel operated at 39°C. Over 500 bases sequence generated 50 min.
elsevier.com
sci-hub.se 参考文章(19)
S Tabor, C C Richardson, DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Effect of pyrophosphorolysis and metal ions. Journal of Biological Chemistry. ,vol. 265, pp. 8322- 8328 ,(1990) , 10.1016/S0021-9258(19)39075-1
Harold Swerdlow, Shaole Wu, Heather Harke, Norman J. Dovichi, Capillary gel electrophoresis for DNA sequencing : Laser-induced fluorescence detection with the sheath flow cuvette Journal of Chromatography A. ,vol. 516, pp. 61- 67 ,(1990) , 10.1016/S0021-9673(01)90204-3
Lloyd M. Smith, Jane Z. Sanders, Robert J. Kaiser, Peter Hughes, Chris Dodd, Charles R. Connell, Cheryl Heiner, Stephen B. H. Kent, Leroy E. Hood, Fluorescence detection in automated DNA sequence analysis Nature. ,vol. 321, pp. 674- 679 ,(1986) , 10.1038/321674A0
Wilhelm Ansorge, Brian S. Sproat, Josef Stegemann, Christian Schwager, A non-radioactive automated method for DNA sequence determination. Journal of Biochemical and Biophysical Methods. ,vol. 13, pp. 315- 323 ,(1986) , 10.1016/0165-022X(86)90038-2
DaYong Chen, Heather R. Harke, Norman J. Dovichi, Two-label peak-height encoded DNA sequencing by capillary gel electrophoresis: three examples Nucleic Acids Research. ,vol. 20, pp. 4873- 4880 ,(1992) , 10.1093/NAR/20.18.4873
Stephen L. Pentoney, Kenneth D. Konrad, Wilbur Kaye, A single-fluor approach to DNA sequence determination using high performance capillary electrophoresis. Electrophoresis. ,vol. 13, pp. 467- 474 ,(1992) , 10.1002/ELPS.11501301100
Harold. Swerdlow, Jian Zhong. Zhang, Da Yong. Chen, Heather R. Harke, Ronda. Grey, Shaole. Wu, Norman J. Dovichi, Carl. Fuller, Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence. Analytical Chemistry. ,vol. 63, pp. 2835- 2841 ,(1991) , 10.1021/AC00024A006
Howard. Drossman, John A. Luckey, Anthony J. Kostichka, Jonathan. D'Cunha, Lloyd M. Smith, High-speed separations of DNA sequencing reactions by capillary electrophoresis Analytical Chemistry. ,vol. 62, pp. 900- 903 ,(1990) , 10.1021/AC00208A003
Lyle R. Middendorf, John C. Bruce, Robert C. Bruce, Robert D. Eckles, Daniel L. Grone, Stephen C. Roemer, Gary D. Sloniker, David L. Steffens, Scott L. Sutter, John A. Brumbaugh, Gabor Patonay, Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus. Electrophoresis. ,vol. 13, pp. 487- 494 ,(1992) , 10.1002/ELPS.11501301103
Achim E. Karger, Joel M. Harris, Raymond F. Gesteland, Multiwavelength fluorescence detection for DNA sequencing using capillary electrophoresis Nucleic Acids Research. ,vol. 19, pp. 4955- 4962 ,(1991) , 10.1093/NAR/19.18.4955