A STUDY OF THE BIOTECHNOLOGICAL APPLICATIONS OF NOVOSPHINGOBIUM PUTEOLANUM PP1Y.

作者: Luca Troncone

DOI: 10.6092/UNINA/FEDOA/8892

关键词: Environmental pollutionEnergy sourceXenobioticStrain (chemistry)BioremediationPropylbenzenePyreneChemistryOrganic chemistryNovosphingobium

摘要: Environmental pollution caused by the release of a wide range xenobiotic compounds has assumed serious proportions. Bioremediation techniques, utilizing microorganisms to reduce concentration and toxicity various chemical pollutants such as petroleum hydrocarbons, polycyclic aromatic polychlorinated biphenyls, industrial solvents pesticides, are most promising strategies for restoration polluted environments. Several bacterial strains with degradative abilities have already been isolated characterized. However, full exploitation potential bioremediation requires not only isolation large number but also an accurate characterization these both at microbiological biochemical/genetic level. This knowledge is necessary perform rational planning interventions. The present work describes new able degrade hydrocarbons directly from environments means appropriate selection procedures. Novosphingobium sp. PP1Y, surface seawater sample collected closed bay in harbor Pozzuoli (Naples, Italy), uses fuels its sole carbon energy source. Like some other Sphingomonads, this strain can grow either planktonic free cells or sessile-aggregated flocks. In addition, was found biofilm on several types solid liquid hydrophobic surfaces including polystyrene, polypropylene diesel oil. Strain PP1Y pure alkanes alkane mixtures surprisingly mono, bi, tri tetracyclic heterocyclic compounds. During growth oil, organic layer emulsified resulting formation small biofilm-coated drops, whereas during dissolved paraffin oil drops coated if contain selected compounds, like pyrene, propylbenzene, tetrahydronaphthalene compounds. These peculiar characteristics suggest adapted efficiently water/fuel interface using fraction source. The whole genome sequence could provide clues about metabolism species possibility manipulating it purposes. The comprised single 3.9 Mbp circular chromosome 3 plasmids, one megaplasmid (Mpl; 1.16 Mbp), plasmid (Lpl; 0.19 (Spl; 0.05 Mbp). Notably, first time that size identified sphingomonad. Our results will help clarify molecular basis unusual features engineer enhanced cultural abilities.

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