Granulocyte-macrophage colony-stimulating factor, phorbol ester, and sodium butyrate induce the CD11c integrin gene promoter activity during myeloid cell differentiation

摘要: To analyze the activity of CD11c promoter during myeloid differentiation without limitations transient expression systems, we have stably transfected U937 cell line with pCD11C361-Luc plasmid, in which firefly luciferase cDNA is driven by region -361/+43, previously shown to confer specificity reporter genes. The stable transfectants (U937-C361) retained ability differentiate response phorbol-ester (PMA), sodium butyrate (SB), granulocyte- macrophage colony-stimulating factor (GM-CSF), and other differentiating agents. U937-C361 correlated increased cellular levels, showing inducibility establishing U937- C361 cells as a suitable system for studying differentiation-inducing capacity cytokines, growth, factors, biological modifiers. Unexpectedly, gene showed distinct kinetics magnitude on PMA-, SB-, GM-CSF-triggered differentiation. Moreover, SB synergized either PMA or GM-CSF enhancing both surface p150,95 cells. Furthermore, existence c-Myb-binding site at - 85, importance -99/-61 PMA- SB-triggered differentiation, dependency responsiveness an intact AP-1-binding located -60. These results, together lack functional effect mutations disrupting Sp1-and Myb-binding sites within proximal promoter, indicate that pathways indicated phorbol esters (or GM-CSF) activate set transcription factors show differentiation-inducibility maps -99/-53 promoter.