Characterization of the myeloid-specific CD11b promoter

摘要: The CD11b/CD18 heterodimeric surface antigen is expressed exclusively on human monocytes, macrophages, granulocytes, and natural killer cells. During differentiation of myeloid cell lines, CD11b steady state messenger RNA levels increase significantly; we show here that transcription rates commensurately. A 1.7-kb fragment 5' flanking sequence directs expression a reporter gene specifically in lines. Deletion analysis localizes elements directing high tissue-specific to the 412 bp proximal transcriptional start site. This contains two consensus binding sites for Sp1, GATA motif, purine-rich presents potential members ets family genes. Analysis this promoter should result isolation myeloid-specific factors development methods direct heterologous