Contact inhibition of locomotion and mechanical cross-talk between cell–cell and cell–substrate adhesion determine the pattern of junctional tension in epithelial cell aggregates

摘要: We used a computational approach to analyze the biomechanics of epithelial cell aggregates-islands, stripes, or entire monolayers-that combines both vertex and contact-inhibition-of-locomotion models include cell-cell cell-substrate adhesion. Examination distribution protrusions (adhesion substrate) in model predicted high-order profiles organization that agree with those previously seen experimentally. Cells acquired an asymmetric basal protrusions, traction forces, apical aspect ratios decreased when moving from edge island center. Our silico analysis also showed tension on junctions stress is not homogeneous across island. Instead, these parameters are higher at center scale up size, which we confirmed experimentally using laser ablation assays immunofluorescence. Without formally being three-dimensional model, our has minimal elements necessary reproduce cellular forces mechanical cross-talk, as well principal cells within aggregates. By making testable predictions, can aid tissues, especially local changes and/or adhesion drive collective behavior.