Prefrontal cortex expression of chromatin modifier genes in male WSP and WSR mice changes across ethanol dependence, withdrawal, and abstinence

摘要: Abstract Alcohol-use disorder (AUD) is a relapsing associated with excessive ethanol consumption. Recent studies support the involvement of epigenetic mechanisms in development AUD. Studies carried out so far have focused on few specific modifications. The goal this project was to investigate gene expression changes regulators that mediate broad array chromatin modifications after chronic alcohol exposure, exposure followed by 8 h withdrawal, and 21 days abstinence Withdrawal-Resistant (WSR) Withdrawal Seizure-Prone (WSP) selected mouse lines. We found vapor highly intoxicating levels alters several remodeling genes measured quantitative PCR analyses. identified effects were independent lines, which, however, displayed baseline differences expression. reported dysregulation involved histone acetylation, deacetylation, lysine arginine methylation ubiquitinationhylation during but not abstinence. Ethanol-induced are consistent decreased acetylation deposition permissive ubiquitination mark H2BK120ub, reduced transcription. On other hand, ethanol-induced increased net result these likely depend combination tail present at given time promoter. Since does modulate unidirectionally, it surprising unidirectionally alter structure toward closed or open state, as suggested results study.