Effect of mucosal and systemic immunization with virus‐like particles of severe acute respiratory syndrome coronavirus in mice

摘要: Nasal administration has emerged as a promising and attractive route for vaccination, especially the prophylaxis of respiratory diseases. Our previous studies have shown that severe acute syndrome coronavirus (SARS-CoV) virus-like particles (VLPs) can be assembled using recombinant baculovirus (rBV) expression system such VLPs induce specific humoral cellular immune responses in mice after subcutaneous injection. Here, we investigated mucosal to SARS-CoV mouse model. Mice were immunized parallel, intraperitoneally or intranasally, with alone plus cytosine–phosphate–guanosine (CpG). Immune responses, including production SARS-CoV-specific serum immunoglobulin G (IgG) secretory A (sIgA), determined secretions tissues. Both immunizations induced IgG, although levels IgG groups via intraperitoneal (i.p.) higher. sIgA was detected saliva intranasally but not intraperitoneally. CpG had an adjuvant effect on IgA genital tract washes when administered only affected faeces samples In addition, also tissues from lung intestine, while increased level intestine. Most importantly, neutralization antibodies sera i.p. intranasal (i.n.) immunizations. Secretions i.n. group showed activity. Furthermore, elicited demonstrated by enzyme-linked immunospot (ELISPOT) assay analyses. summary, our study indicates immunization rBV represent effective means eliciting protective systemic against SARS-CoV, providing important information vaccine design.