Reprogramming committed murine blood cells to induced hematopoietic stem cells with defined factors
作者： Jonah Riddell , Roi Gazit , Brian S. Garrison , Guoji Guo , Assieh Saadatpour
DOI: 10.1016/J.CELL.2014.04.006
关键词: Cell biology 、 Haematopoiesis 、 Immunology 、 Endothelial stem cell 、 Hematopoietic stem cell transplantation 、 Myeloid 、 Biology 、 Stem cell 、 Transplantation 、 Reprogramming 、 Progenitor cell
摘要: Hematopoietic stem cells (HSCs) sustain blood formation throughout life and are the functional units of bone marrow transplantation. We show that transient expression six transcription factors Run1t1, Hlf, Lmo2, Prdm5, Pbx1, Zfp37 imparts multilineage transplantation potential onto otherwise committed lymphoid myeloid progenitors effector cells. Inclusion Mycn Meis1 use polycistronic viruses increase reprogramming efficacy. The reprogrammed cells, designated induced-HSCs (iHSCs), possess clonal differentiation potential, reconstitute stem/progenitor compartments, serially transplantable. Single-cell analysis revealed iHSCs derived under optimal conditions exhibit a gene profile is highly similar to endogenous HSCs. These findings demonstrate set defined sufficient activate networks governing HSC identity in Our results raise prospect cell may be strategy for derivation transplantable clinical application.
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Antonius G. Rolink, Stephen L. Nutt, Fritz Melchers, Meinrad Busslinger, Long-term in vivo reconstitution of T-cell development by Pax5-deficient B-cell progenitors Nature. ,vol. 401, pp. 603- 606 ,(1999) , 10.1038/44164
Stephen L. Nutt, Barry Heavey, Antonius G. Rolink, Meinrad Busslinger, Commitment to the B-lymphoid lineage depends on the transcription factor Pax5 Nature. ,vol. 401, pp. 556- 562 ,(1999) , 10.1038/44076
Huafeng Xie, Min Ye, Ru Feng, Thomas Graf, Stepwise Reprogramming of B Cells into Macrophages Cell. ,vol. 117, pp. 663- 676 ,(2004) , 10.1016/S0092-8674(04)00419-2
Catherine V. Laiosa, Matthias Stadtfeld, Huafeng Xie, Luisa de Andres-Aguayo, Thomas Graf, Reprogramming of Committed T Cell Progenitors to Macrophages and Dendritic Cells by C/EBPα and PU.1 Transcription Factors Immunity. ,vol. 25, pp. 731- 744 ,(2006) , 10.1016/J.IMMUNI.2006.09.011
Fatih Kocabas, Junke Zheng, Suwannee Thet, Neal G. Copeland, Nancy A. Jenkins, Ralph J. DeBerardinis, Chengcheng Zhang, Hesham A. Sadek, Meis1 regulates the metabolic phenotype and oxidant defense of hematopoietic stem cells Blood. ,vol. 120, pp. 4963- 4972 ,(2012) , 10.1182/BLOOD-2012-05-432260
H. Iwasaki, S.-i. Mizuno, Y. Arinobu, H. Ozawa, Y. Mori, H. Shigematsu, K. Takatsu, D. G. Tenen, K. Akashi, The order of expression of transcription factors directs hierarchical specification of hematopoietic lineages Genes & Development. ,vol. 20, pp. 3010- 3021 ,(2006) , 10.1101/GAD.1493506
Tom Taghon, Mary A Yui, Ellen V Rothenberg, Mast cell lineage diversion of T lineage precursors by the essential T cell transcription factor GATA-3 Nature Immunology. ,vol. 8, pp. 845- 855 ,(2007) , 10.1038/NI1486
J. Choi, M. L. Costa, C. S. Mermelstein, C. Chagas, S. Holtzer, H. Holtzer, MyoD converts primary dermal fibroblasts, chondroblasts, smooth muscle, and retinal pigmented epithelial cells into striated mononucleated myoblasts and multinucleated myotubes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 7988- 7992 ,(1990) , 10.1073/PNAS.87.20.7988
Zeenath Unnisa, Jason P. Clark, Jayeeta Roychoudhury, Elizabeth Thomas, Lino Tessarollo, Neal G. Copeland, Nancy A. Jenkins, H. Leighton Grimes, Ashish R. Kumar, Meis1 preserves hematopoietic stem cells in mice by limiting oxidative stress Blood. ,vol. 120, pp. 4973- 4981 ,(2012) , 10.1182/BLOOD-2012-06-435800
César Cobaleda, Wolfram Jochum, Meinrad Busslinger, Conversion of mature B cells into T cells by dedifferentiation to uncommitted progenitors Nature. ,vol. 449, pp. 473- 477 ,(2007) , 10.1038/NATURE06159