Viscosity of Plasma as a Key Factor in Assessment of Extracellular Vesicles by Light Scattering.
作者： Božič , Sitar , Junkar , Štukelj , Pajnič
DOI: 10.3390/CELLS8091046
关键词: Blood plasma 、 Plasma 、 Viscosity 、 Dynamic light scattering 、 Suspension (chemistry) 、 Light scattering 、 Vesicle 、 Static light scattering 、 Materials science 、 Biophysics
摘要: Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes compositions of harvested missing. A plausible model description EV isolates has not been developed. Furthermore, identity genesis EVs still obscure relevant parameters have yet identified. The purpose this work is to better understand mechanisms taking place during harvesting EVs, particular role viscosity suspension. were blood plasma by repeated centrifugation washing samples. Their size shape assessed using combination static dynamic light scattering. average parameter particles was found be ρ ~ 1 (0.94–1.1 exosome standards 0.7–1.2 isolates), pertaining spherical shells (spherical vesicles). This study estimated value coefficient medium 1.2 mPa/s. It can concluded scattering could method upon considering with transient identity.
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