Molecular heterogeneity of Rhipicephalus sanguineus sensu lato and screening for Ehrlichia canis in mainland Portugal
作者： Gustavo S. Sanches , Joana Couto , Rita Silva-Pedrosa , Joana Ferrolho , Ana Sofia Santos
DOI: 10.1016/J.TTBDIS.2018.06.002
关键词: Genetic distance 、 Lineage (genetic) 、 Rhipicephalus sanguineus 、 Sensu 、 Biology 、 Zoology 、 Tick-borne disease 、 Tick 、 Ehrlichia canis 、 Canis
摘要: The present study aimed to expand knowledge regarding the molecular characterization of R. sanguineus s.l. in Portugal and screen for ticks naturally infected with E. canis. A total 113 were collected questing or attached domestic wild animals from 18 administrative regions mainland Portugal. All analyzed 16S rRNA partial sequences obtained showed high genetic similarities specimens belonging temperate lineage. These revealed eight haplotypes (H1‒H8), a distance ranging 0.3% 1.4%. convenience sample representing approximately 75% all was tested presence canis by qPCR dsb gene. No found be this pathogen. Accordingly, further studies are required determine role lineage maintenance transmission, as well elucidate if different other tick species act vectors dogs
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