ENHANCED CUTANEOUS WOUND HEALING BY THE LEAF EXTRACT OF ACHILLEA ERIOPHORA D.C. USING THE IN VITRO SCRATCH ASSAY

摘要: Achillea eriophora D.C. (Asteraceae), an endemic species in Iran, is used extensively Iranian folk medicine to treat various ailments, including gastrointestinal disorders, skin inflammations and wound healing. The present study was aimed investigate the effects of methanolic extract of  A. leaves on stimulation human fibroblast cell proliferation migration as two pivotal stages healing process. Methanolic prepared by maceration method. Total phenol flavonoid contents were measured using spectrophotometry. Cytotoxic evaluated MTT assay cells. Moreover, treated cells assessed closure a denuded area, made scratching confluent monolayer cultures.  The results of cytotoxicity assay indicated that did not have any cytotoxic effect when at concentrations up 2 µg/mL. Human stimulated low (0.1-0.8 µg/mL), highest level observed lowest treatment (0.1 µg/mL). Migration induced intermediate (1-30 µg/mL) most effective dose estimated 1 Due fact lower concentration extract, which showed best stimulatory effects, toxic cells, crude from could be recommended potential source for improving activity skin.