Nanoscopy in a living multicellular organism expressing GFP.
作者： Brian R. Rankin , Gael Moneron , Christian A. Wurm , Jessica C. Nelson , Arne Walter
DOI: 10.1016/J.BPJ.2011.05.020
关键词: Saccharomyces cerevisiae 、 Caenorhabditis elegans 、 Green fluorescent protein 、 Fluorescence microscope 、 STED microscopy 、 Cell biology 、 Resolution (electron density) 、 Microscopy 、 Biology 、 Multicellular organism
摘要: We report superresolution fluorescence microscopy in an intact living organism, namely Caenorhabditis elegans nematodes expressing green fluorescent protein (GFP)-fusion proteins. also superresolve, by stimulated emission depletion (STED) microscopy, cultured cells, demonstrating that STED with GFP can be widely applied. performed both pulsed and continuous-wave lasers spanning a wide wavelength range from at least 556-592 nm. Acquiring subdiffraction resolution images within seconds enables the recording of movies revealing structural dynamics. These results demonstrate numerous studies live samples employing as marker sub- diffraction resolution.
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sci-hub.se 参考文章(12)
Jun-ichi Hotta, Eduard Fron, Peter Dedecker, Kris P. F. Janssen, Chen Li, Klaus Müllen, Benjamin Harke, Johanna Bückers, Stefan W. Hell, Johan Hofkens, Spectroscopic Rationale for Efficient Stimulated-Emission Depletion Microscopy Fluorophores Journal of the American Chemical Society. ,vol. 132, pp. 5021- 5023 ,(2010) , 10.1021/JA100079W
Qifeng Li, Sherry S.H. Wu, Keng C. Chou, Subdiffraction-limit two-photon fluorescence microscopy for GFP-tagged cell imaging. Biophysical Journal. ,vol. 97, pp. 3224- 3228 ,(2009) , 10.1016/J.BPJ.2009.09.038
Michael J Rust, Mark Bates, Xiaowei Zhuang, Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) Nature Methods. ,vol. 3, pp. 793- 796 ,(2006) , 10.1038/NMETH929
Katrin I Willig, Robert R Kellner, Rebecca Medda, Birka Hein, Stefan Jakobs, Stefan W Hell, None, Nanoscale resolution in GFP-based microscopy Nature Methods. ,vol. 3, pp. 721- 723 ,(2006) , 10.1038/NMETH922
Brian R Rankin, Stefan W Hell, STED microscopy with a MHz pulsed stimulated-Raman-scattering source Optics Express. ,vol. 17, pp. 15679- 15684 ,(2009) , 10.1364/OE.17.015679
E. Betzig, G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, H. F. Hess, Imaging intracellular fluorescent proteins at nanometer resolution. Science. ,vol. 313, pp. 1642- 1645 ,(2006) , 10.1126/SCIENCE.1127344
Claes Axäng, Manish Rauthan, David H Hall, Marc Pilon, Developmental genetics of the C. elegans pharyngeal neurons NSML and NSMR. BMC Developmental Biology. ,vol. 8, pp. 38- 38 ,(2008) , 10.1186/1471-213X-8-38
Samuel T Hess, Thanu PK Girirajan, Michael D Mason, None, Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy Biophysical Journal. ,vol. 91, pp. 4258- 4272 ,(2006) , 10.1529/BIOPHYSJ.106.091116
Stefan W. Hell, Jan Wichmann, Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy Optics Letters. ,vol. 19, pp. 780- 782 ,(1994) , 10.1364/OL.19.000780
Peter Kner, Bryant B Chhun, Eric R Griffis, Lukman Winoto, Mats G L Gustafsson, Super-Resolution Video Microscopy of Live Cells by Structured Illumination Nature Methods. ,vol. 6, pp. 339- 342 ,(2009) , 10.1038/NMETH.1324