Spectroscopic Rationale for Efficient Stimulated-Emission Depletion Microscopy Fluorophores

作者: Jun-ichi Hotta , Eduard Fron , Peter Dedecker , Kris P. F. Janssen , Chen Li

DOI: 10.1021/JA100079W

关键词:

摘要: We report a rationale for identifying superior dyes for stimulated-emission depletion (STED) microscopy. We compared the dyes pPDI and pTDI, which displayed excellent photostability …

参考文章(30)
Mike Heilemann, Sebastian van de Linde, Mark Schüttpelz, Robert Kasper, Britta Seefeldt, Anindita Mukherjee, Philip Tinnefeld, Markus Sauer, Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes† Angewandte Chemie. ,vol. 47, pp. 6172- 6176 ,(2008) , 10.1002/ANIE.200802376
G. Donnert, J. Keller, R. Medda, M. A. Andrei, S. O. Rizzoli, R. Luhrmann, R. Jahn, C. Eggeling, S. W. Hell, Macromolecular-scale resolution in biological fluorescence microscopy. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 103, pp. 11440- 11445 ,(2006) , 10.1073/PNAS.0604965103
Philip Tinnefeld, Johan Hofkens, Dirk-Peter Herten, Sadahiro Masuo, Tom Vosch, Mircea Cotlet, Satoshi Habuchi, Klaus Müllen, Frans C. De Schryver, Markus Sauer, Higher-excited-state photophysical pathways in multichromophoric systems revealed by single-molecule fluorescence spectroscopy ChemPhysChem. ,vol. 5, pp. 1786- 1790 ,(2004) , 10.1002/CPHC.200400325
Gerald Donnert, Christian Eggeling, Stefan W. Hell, Triplet-relaxation microscopy with bunched pulsed excitation Photochemical and Photobiological Sciences. ,vol. 8, pp. 481- 485 ,(2009) , 10.1039/B903357M
Hiroshi Uji-i, Sergey M. Melnikov, Ania Deres, Giacomo Bergamini, Frans De Schryver, Andreas Herrmann, Klaus Müllen, Jörg Enderlein, Johan Hofkens, Visualizing spatial and temporal heterogeneity of single molecule rotational diffusion in a glassy polymer by defocused wide-field imaging Polymer. ,vol. 47, pp. 2511- 2518 ,(2006) , 10.1016/J.POLYMER.2005.11.094
Eduard Fron, Roberto Pilot, Gerd Schweitzer, Jianqiang Qu, Andreas Herrmann, Klaus Müllen, Johan Hofkens, Mark Van der Auweraer, Frans C. De Schryver, Photoinduced electron-transfer in perylenediimide triphenylamine-based dendrimers: single photon timing and femtosecond transient absorption spectroscopy. Photochemical and Photobiological Sciences. ,vol. 7, pp. 597- 604 ,(2008) , 10.1039/B718479D
Rayleigh, XV. On the theory of optical images, with special reference to the microscope Philosophical Magazine Series 1. ,vol. 42, pp. 167- 195 ,(2009) , 10.1080/14786449608620902
M. Heilemann, P. Dedecker, J. Hofkens, M. Sauer, Photoswitches: Key molecules for subdiffraction-resolution fluorescence imaging and molecular quantification Laser & Photonics Reviews. ,vol. 3, pp. 180- 202 ,(2009) , 10.1002/LPOR.200810043
Robert H Webb, Confocal optical microscopy Reports on Progress in Physics. ,vol. 59, pp. 427- 471 ,(1996) , 10.1088/0034-4885/59/3/003