X-Ray studies reveal lanthanide binding sites at the A/B5 interface of E. coli heat labile enterotoxin
作者： Titia K. Sixma , Anke C. Terwisscha van Scheitinga , Kor H. Kalk , Kangjing Zhou , Ellen S. Wartna
DOI: 10.1016/0014-5793(92)80355-K
关键词: Enterotoxin 、 Samarium 、 Cholera toxin 、 Lanthanide 、 Crystallography 、 Heat-labile enterotoxin 、 Pentamer 、 Ion binding 、 Chemistry 、 Binding site
摘要: The crystal structure determination of heat labile enterotoxin (LT) bound to two different lanthanide ions, erbium and samarium, revealed distinct ion binding sites in the interface A subunit B pentamer toxin. One is conserved very similar cholera toxin sequence. These may be potential calcium sites. Erbium samarium causes a change LT: rotation A1 up degrees relative pentamer.
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sci-hub.se 参考文章(20)
J Moss, J C Osborne, P.H. Fishman, S Nakaya, D.C. Robertson, Escherichia coli Heat-labile Enterotoxin GANGLIOSIDE SPECIFICITY AND ADP-RIBOSYLTRANSFERASE ACTIVITY * Journal of Biological Chemistry. ,vol. 256, pp. 12861- 12865 ,(1981) , 10.1016/S0021-9258(18)42975-4
Timothy J. Foster, P. Owen, Immunochemical and Molecular Genetic Analysis of Bacterial Pathogens ,(1988)
J J Mekalanos, R J Collier, W R Romig, Enzymic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction, and subunit composition. Journal of Biological Chemistry. ,vol. 254, pp. 5855- 5861 ,(1979) , 10.1016/S0021-9258(18)50491-9
T Yamamoto, T Gojobori, T Yokota, Evolutionary origin of pathogenic determinants in enterotoxigenic Escherichia coli and Vibrio cholerae O1. Journal of Bacteriology. ,vol. 169, pp. 1352- 1357 ,(1987) , 10.1128/JB.169.3.1352-1357.1987
S Kassis, J Hagmann, P H Fishman, P P Chang, J Moss, Mechanism of action of cholera toxin on intact cells. Generation of A1 peptide and activation of adenylate cyclase. Journal of Biological Chemistry. ,vol. 257, pp. 12148- 12152 ,(1982) , 10.1016/S0021-9258(18)33691-3
G J Leitch, M S Amer, Lanthanum inhibition of Vibrio cholerae and Escherichia coli enterotoxin-induced enterosorption and its effects on intestinal mucosa cyclic adenosine 3',5'-monophosphate and cyclic guanosine 3',5'-monophosphate levels. Infection and Immunity. ,vol. 11, pp. 1038- 1044 ,(1975) , 10.1128/IAI.11.5.1038-1044.1975
S E Pronk, H Hofstra, H Groendijk, J Kingma, M B Swarte, F Dorner, J Drenth, W G Hol, B Witholt, Heat-labile enterotoxin of Escherichia coli. Characterization of different crystal forms. Journal of Biological Chemistry. ,vol. 260, pp. 13580- 13584 ,(1985) , 10.1016/S0021-9258(17)38763-X
R. J. Read, Improved Fourier coefficients for maps using phases from partial structures with errors Acta Crystallographica Section A. ,vol. 42, pp. 140- 149 ,(1986) , 10.1107/S0108767386099622
D. D. Maenz, S. E. Gabriel, G. W. Forsyth, Calcium transport affinity, ion competition and cholera toxin effects on cytosolic Ca concentration. The Journal of Membrane Biology. ,vol. 96, pp. 243- 249 ,(1987) , 10.1007/BF01869306
A. Messerschmidt, J. W. Pflugrath, Crystal orientation and X-ray pattern prediction routines for area-detector diffractometer systems in macromolecular crystallography Journal of Applied Crystallography. ,vol. 20, pp. 306- 315 ,(1987) , 10.1107/S002188988708662X