Furthering the state of knowledge on the electric properties of hemi-ellipsoidal single cells and cell patches on electrodes.

摘要: The impedance of electrodes with adherent biological cells correlates cell viability and proliferation. To model this correlation, we exploited the idea that introduction a highly conductive layer into equatorial equipotential slice system an oriented, freely suspended, single ellipsoidal may split mirror-symmetrical halves without changing field distribution. Each half possesses system's contains hemiellipsoidal attached to layer, which can be considered bottom electrode. For (ACM) standard electrical properties for external cellular media, assumption membrane subcellular cleft in 100 nm range, as found cells, changed potential distribution over one-% range up frequencies 1 MHz. simplicity, distributions slices spheroidal objects numerically calculated 2D. 2D converted three dimensions using simplified equations influential radii spheroids. After ACM approach was expanded patch models (APMs), feasibility our modifications tested two criteria: constancy plane touching poles ACMs or APMs comparison impedance, could from overall current between electrode plane-parallel counter-electrode, suspension obtained Maxwell-Wagner's mixing equation applied cells.