Development and application of an antibody ELISA for the marker protein of ovine pulmonary carcinoma
作者： Jimmy Kwang , Jim Keen , Sergio Rosati , Francesco Tolari
DOI: 10.1016/0165-2427(94)05409-L
关键词: Virology 、 Serology 、 Immunoassay 、 Antibody 、 Biology 、 Recombinant DNA 、 Virus 、 Escherichia coli 、 Retrovirus 、 Subclinical infection
摘要: Ovine pulmonary carcinoma (OPC) is a contagious neoplasia with suspected retroviral etiology. The major core protein (P27) of the putative OPC virus cross-reacts antibodies to P27 Mason-Pfizer monkey (MPMV), type-D retrovirus. This serological reactivity serves as only accepted biological marker for OPC. In order make useful reagent detection serodiagnosis and epidemiological studies, MPMV-P27 coding region was cloned expressed in Escherichia coli. Gel purified recombinant used develop an immunoassay. enzyme-linked immunosorbent assay (ELISA) then screen 223 sera from US sheep 176 Italian sheep. this study, we found: (1) high prevalence infection retrovirus chronic pneumonia; (2) subclinical may be more common than indicated by rare recorded occurrence carcinoma; (3) apparent association between ovine lentivirus (OLV) infection.
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