Analysis of a Type D Retroviral Capsid Gene Expressed in Ovine Pulmonary Carcinoma and Present in Both Affected and Unaffected Sheep Genomes

作者: Steven J. Hecht , Jonathan O. Carlson , James C. Demartini

DOI: 10.1006/VIRO.1994.1366

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摘要: Ovine pulmonary carcinoma is a contagious neoplastic disease of sheep which can be transmitted experimentally with cell-free filtrate. Products homologous to structural proteins type D and B retroviruses are expressed in the tumors, but an infectious retrovirus has yet isolated. Retroviral RNA was isolated from affected Peruvian used as substrate for reverse transcription. The DNA obtained subjected polymerase chain reaction amplification using degenerate primers designed amino acid sequences shared by capsid peptides viruses that cross-react found tumor tissues. A retroviral region lineage proved highly jaagsiekte South Africa. This sequence detect virus genomes without ovine carcinoma. More than 20 endogenous hybridized probe were examined, assortment these could followed families. Initial experiments did not show any obvious differences viral presence tumors compared background unaffected lungs.

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