Induction and growth of adventitious roots and bioreactor culture in Codonopsis lanceolata
作者： Chang-Ho Ahn , Kee-Hwa Bae , Jae-Seon Yi , Yong-Eui Choi
DOI: 10.5010/JPB.2008.35.2.155
关键词: Botany 、 Biology 、 Shake-Flask Culture 、 Codonopsis lanceolata 、 Explant culture 、 Murashige and Skoog medium 、 Bioreactor 、 Solid medium 、 Fresh weight 、 Auxin
摘要: This paper reported the establishment of mass production system adventitious roots Codonopsis lanceolata through shake flask and bioreactor culture. Induction was started from explants leaf, stem root on 1/2 strength Murashing Skoog (MS) solid medium. Stem segments were best for induction compared to leaf segments. Among different auxins tested (NAA, IBA IAA), number per explant highest medium with 1.0 mg/L IBA, produced explant. However, growth fast in presence at low concentration (0.1 mg/L). In culture, maximum (fresh weight) obtained half-strength MS full-strength one-third When shake-flask culture transferred 5 L air-lift bioreactor, 16 times fresh weight increase gained after one month These results indicate that this protocol C. can be applied large scale practical application.
koreascience.or.kr参考文章(5)
S.M.A. Zobayed, P.K. Saxena, In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John's wort (Hypericum perforatum L. cv ‘New Stem’) in a temporary immersion bioreactor Plant Science. ,vol. 165, pp. 463- 470 ,(2003) , 10.1016/S0168-9452(03)00064-5
Mitsuyasu Ushijima, Noriko Komoto, Yoshimi Sugizono, Ikuko Mizuno, Masanori Sumihiro, Makoto Ichikawa, Minoru Hayama, Nobuo Kawahara, Takahisa Nakane, Osamu Shirota, Setsuko Sekita, Masanori Kuroyanagi, Triterpene Glycosides from the Roots of Codonopsis lanceolata Chemical & Pharmaceutical Bulletin. ,vol. 56, pp. 308- 314 ,(2008) , 10.1248/CPB.56.308
F. Bourgaud, A. Gravot, S. Milesi, E. Gontier, Production of plant secondary metabolites: a historical perspective Plant Science. ,vol. 161, pp. 839- 851 ,(2001) , 10.1016/S0168-9452(01)00490-3
Kee-Won Yu, Wenyuan Gao, Eun-Joo Hahn, Kee-Yoeup Paek, Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer Biochemical Engineering Journal. ,vol. 11, pp. 211- 215 ,(2002) , 10.1016/S1369-703X(02)00029-3
A Lazaridou, T Roukas, C.G Biliaderis, H Vaikousi, Characterization of pullulan produced from beet molasses by Aureobasidium pullulans in a stirred tank reactor under varying agitation Enzyme and Microbial Technology. ,vol. 31, pp. 122- 132 ,(2002) , 10.1016/S0141-0229(02)00082-0