In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John's wort (Hypericum perforatum L. cv ‘New Stem’) in a temporary immersion bioreactor

摘要: Abstract Hypericum perforatum L. (St. John's wort) is a traditional medicinal plant, which has been used for the treatment of neurological disorders and depression. In vitro regeneration St. wort provides one approach mass clonal propagation this facultative apomictic species. The current study was designed (i) to compare potential several explant types (ii) investigate use root explants large-scale production in bioreactors. Root responded better than shoot tip, leaf, hypocotyl or stem terms thidiazuron-induced organogenesis. efficacy different auxins (indoleacetic acid (IAA), indolebutyric naphthaleneacetic acid) induction de novo organogenesis from biomass accumulation were evaluated. All tested effectively induced new roots, 32 roots per explant, with an optimal response medium containing 5 μM IAA. Three bioreactors, airlift bioreactor, temporary immersion bioreactor (TI bioreactor) Erlenmeyer flask, compared efficiency TI shoots elongated faster comparison other treatments. Transplanting ex acclimatization period 7 days sufficient promote establishment plants greenhouse more 95% regenerated survived. results show that can be efficiently variety bioreactors aseptic bioactive compounds.