Comparisons with Caenorhabditis (∼100 Mb) and Drosophila (∼175 Mb) Using Flow Cytometry Show Genome Size in Arabidopsis to be ∼157 Mb and thus ∼25 % Larger than the Arabidopsis Genome Initiative Estimate of ∼125 Mb

摘要: Recent genome sequencing papers have given sizes of 180 Mb for Drosophila melanogaster Iso-1 and 125 Arabidopsis thaliana Columbia. The former agrees with early cytochemical estimates, but numerous cytometric estimates around 170 imply that a size arabidopsis is an underestimate. In this study, nuclei species pairs were compared directly using flow cytometry. Co-run Columbia female gave 2C peak only approx. 15 % below drosophila, 16C endopolyploid had more DNA than chicken (with >2280 Mb). Caenorhabditis elegans Bristol N2 (genome 100 Mb) co-run or drosophila 75 above C. elegans, 57 elegans. This confirms 1C in 175 and, combined other evidence, leads us to conclude the not Mb, probably 157 Mb. It likely discrepancy represents extra repeated sequences unsequenced gaps heterochromatic regions. Complete until no remain at telomeres, nucleolar organizing regions centromeres still needed provide first precise angiosperm C-value as benchmark calibration standard plant genomes, ensure genes been missed arabidopsis, especially centromeric regions, which are clearly larger once imagined.