Surveillance and molecular characterization of human sapovirus in patients with acute gastroenteritis in Brazil, 2010 to 2017.

摘要: Abstract Background Human sapoviruses (HuSaV) are associated with acute gastroenteritis (AGE), causing sporadic cases and outbreaks in patients worldwide. In Brazil, however, there few reports describing the prevalence of HuSaV AGE. Objective Describing diversity Brazil by detecting molecularly characterizing among AGE during an 8-year period (2010–2017). Study design A total 3974 stool samples, testing negative for rotavirus (RVA), norovirus (NoV) human adenovirus (HAdV), were selected screened presence HuSaV. Nested RT-PCR performed a partial region VP1, sequenced genetic analyzed genotyping positive samples. Results current study, was determined to be 3.7% (149/3974). higher prevalence, 5.7% (118/2074), observed children under 2 years age. During surveillance period, 13 detected: 12 3 old one outbreak adults. Among 149 cases, 106 samples (71%) successfully sequenced. The most prevalent genotype found GI.1 (44.3%), followed GI.2 (21.7%), GI.3 (3.8%), GI.6 (2.8%), GII.1 (5.7%), GII.2 (8.5%), GII.3 GII.4 GII.5 (5.7%) GIV.1 (1.9%). Two strains characterized this study are, date, only reported Brazil. Conclusions present elucidated circulation highlight that has not assumed epidemiological importance country after introduction RVA vaccine.