A murine model of granulomatous colitis with mesenteric lymphadenitis induced by mycobacterial cord factor.

摘要: Granulomatous colitis is a major entity of human intestinal diseases. We previously reported that intravenous injection mycobacterial cord factor (CF), potent macrophage activator, induced pulmonary granulomas in mice with enhanced production Th1 cytokines and chemokines. In this study we made murine model granulomatous by intramural CF. A single dose 300 µg CF was injected into the wall rat mouse colon form liposomes. After 1 week developed at site, extending from subserosa to lamina propria, persisted for longer than 6 weeks. They were composed mainly ED1-positive macrophages, which often underwent apoptosis, CD4+ CD8+ lymphocytes, preferentially infiltrated around accumulation. Myofibroblast proliferation not prominent, no appreciable fibrosis resulted after decline granulomas. Although epithelium involved inflammation, tissue injuries such as mucosal erosion or ulceration induced. When formed near Peyer's patches, they invaded deeply lymphoid tissue, producing many small islands. The mesenteric lymph nodes also had islands cortex medulla, but liver spleen displayed changes, suggesting liposomal spreads via lymphatic vessels site. CF-induced colonic associated lymphadenitis will be useful investigating colitis.