Photobiological transformation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) using Rhodobacter sphaeroides

摘要: Abstract Pump-and-treat strategies for groundwater containing explosives may be necessary when the contaminated water approaches sensitive receptors. This project investigated bacterial photosynthesis as a strategy ex situ treatment, using light primary energy source to facilitate RDX transformation. The objective was characterize ability of photosynthetic Rhodobacter sphaeroides (strain ATCC ® 17023 ™) transform high-energy explosive RDX. R. sphaeroides transformed 30 μM within 40 h under conditions; not fully in dark (non-photosynthetic conditions), suggesting that electron transfer mechanism. Experiments with demonstrated succinate and malate were most effective donors photosynthesis, but glycerol also utilized donor. irrespective presence carbon dioxide. shuttling compound anthraquinone-2,6-disulfonate (AQDS) increased transformation kinetics absence CO 2 , cells had excess NADPH needed re-oxidized because there limited fixation. When added, generated more biomass, AQDS no stimulatory effect. End products indicated became soluble, uncharacterized aqueous metabolite, determined 14 C-labeled These data are first suggest photobiological is possible will provide framework which phototrophy can used environmental restoration water.