13C- and 15N-Isotopic Labeling of Proteins

摘要: Originally published in: Molecular Biology in Medicinal Chemistry. Edited by Theodor Dingermann, Dieter Steinhilber and Gerd Folkers. Copyright © 2004 Wiley-VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3-527-30431-8 The sections this article are Introduction Expression Systems for the In Vivo Incorporation of 13C 15N Labels into Proteins Protein Production 15N-labeling E. coli Expression Systems 13C- Proteins P. pastoris 13C- Chinese Hamster Ovary (CHO) Cells 13C- Other Organisms Strategies Selectively 13C- 15N-labeled Proteins Selective Labeling Amino Acids Specific Isotope with 13C Segmental Labeling Cell-free Labeling Components Cell-free Systems Cell-free Techniques Specific Applications Technique Keywords: molecular biology; medicinal chemistry; analysis; 13C- 15N-isotopic labeling proteins; expression systems; in vivo incorporation labels proteins; cell-free isotope labeling; cell-free expression techniques