Chromosome landing at the Mla locus in barley (Hordeum vulgare L.) by means of high-resolution mapping with AFLP markers
作者： G. Schwarz , W. Michalek , V. Mohler , G. Wenzel , A. Jahoor
关键词: Hordeum vulgare 、 Genetics 、 Amplified fragment length polymorphism 、 Bulked segregant analysis 、 Locus (genetics) 、 Yeast artificial chromosome 、 Genetic marker 、 Population 、 Biology 、 Gene mapping
摘要: The complex Mla locus of barley determines resistance to the powdery mildew pathogen Erysiphe graminis f. sp. hordei. With a view towards gene isolation, population consisting 950 F2 individuals derived from cross between near-isogenic lines ‘P01’ (Mla1) and ‘P10’ (Mla12) was used construct high-resolution map region. A fluorescence-based AFLP technique bulked segregant analysis were applied screen for polymorphic, tightly linked markers. Three markers selected as suitable chromosome-landing strategy. One these closely RFLP marker converted into sequence-specific PCR PCR-based screening approximately 70 000 yeast artificial chromosome (YAC) clones revealed three identical YACs harbouring locus. Terminal insert sequences obtained using inverse PCR. STS right YAC end-clone mapped distal
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