Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the tm-2a gene in tomato.

摘要: The Tm-2a gene of tomato confers resistance to the viral pathogen, tobacco mosaic virus. Like many economically important plant genes, has been characterized phenotypically and by classical linkage analysis, yet nothing is known about its product. We report here isolation two DNA clones which are very tightly linked gene. These were identified testing 122 genomic as hybridization probes against Southern blots consisting from pairs nearly isogenic lines with or without Screening such a large number in short period time was facilitated co-labeling simultaneous probing sets up 10 random clones. Tightly distinguished fact that they exhibited one more restriction fragment length polymorphisms between lines. Tight verified segregating F(2) population. Both mapped same locus 0.4 +/- centimorgans away may provide starting points for ;;walk'' this Due availability species, strategy outlined paper should be widely applicable selecting genes interest.