Conformation and aggregation of a polysaccharide: In solution, as transported in Golgi vesicles, and in an extracellular matrix

作者: S. Trachtenberg

DOI: 10.1016/S0889-1605(86)80009-X

关键词: Transmission electron microscopyPolysaccharideScanning transmission electron microscopyExtracellular matrixVesicleBiochemistryScanning electron microscopeGolgi apparatusBiophysicsChemistryMorphology (linguistics)

摘要: The structure of the extracellular matrix polysaccharide (ECMP) Opuntia ficus-indica was studied by transmission and scanning electron microscopy image analysis. In isolated form, in solution, ECMP appears long unbranched. Linear mass measurements indicate that, aggregation chains into bundles occurs. gelling properties at various pHs gels' junction zones were using Alcian blue 8GX as an artificial agent. ECMP-Alcian are electrostatic nature. They constructed from parallel strands cross-bridged molecules with lateral axial repeats 30 35 A, respectively. Similar structures can be formed polygalacturonic, alginic, hyaluronic acids heparin. Parallel studies on polysaccharides morphology, transported Golgi vesicles matrix, carried out thin sections stained for polysaccharides.

参考文章(58)
G.O. Aspinall, Gums and mucilages. Advances in Carbohydrate Chemistry and Biochemistry. ,vol. 24, pp. 333- 379 ,(1969) , 10.1016/S0065-2318(08)60353-4
Marc Cantin, Cell biology of the secretory process Karger. ,(1984)
L Rosenberg, W Hellmann, A K Kleinschmidt, Electron microscopic studies of proteoglycan aggregates from bovine articular cartilage. Journal of Biological Chemistry. ,vol. 250, pp. 1877- 1883 ,(1975) , 10.1016/S0021-9258(19)41776-6
M.D. Walkinshaw, Struther Arnott, Conformations and interactions of pectins: I. X-ray diffraction analyses of sodium pectate in neutral and acidified forms Journal of Molecular Biology. ,vol. 153, pp. 1055- 1073 ,(1981) , 10.1016/0022-2836(81)90467-8
E.R. Morris, D.A. Powell, M.J. Gidley, D.A. Rees, Conformations and interactions of pectins Journal of Molecular Biology. ,vol. 155, pp. 507- 516 ,(1982) , 10.1016/0022-2836(82)90484-3
M.D. Walkinshaw, Struther Arnott, Conformations and interactions of pectins. II. Models for junction zones in pectinic acid and calcium pectate gels. Journal of Molecular Biology. ,vol. 153, pp. 1075- 1085 ,(1981) , 10.1016/0022-2836(81)90468-X
Earl J. Kirkland, B.M. Siegel, N. Uyeda, Y. Fujiyoshi, Improved high resolution image processing of bright field electron micrographs: II. Experiment Ultramicroscopy. ,vol. 17, pp. 87- 103 ,(1985) , 10.1016/0304-3991(85)90002-6
W.G. Whaley, The Golgi Apparatus Cell Biology Monographs. ,(1975) , 10.1007/978-3-7091-7670-2
Shlomo Trachtenberg, Alfred M. Mayer, Biophysical properties of Opuntia ficus-indica mucilage Phytochemistry. ,vol. 21, pp. 2835- 2843 ,(1980) , 10.1016/0031-9422(80)85052-7