Detection of Colonization by Carbapenemase-Producing Gram-Negative Bacilli in Patients by Use of the Xpert MDRO Assay

摘要: ABSTRACT Detecting colonization of patients with carbapenemase-producing bacteria can be difficult. This study compared the sensitivity and specificity a PCR-based method (Xpert MDRO) for detecting bla KPC , NDM VIM carbapenem resistance genes using GeneXpert cartridges to results culture without broth enrichment step on 328 rectal, perirectal, stool samples. The included direct inoculation MacConkey agar plate which 10-μg meropenem disk was placed plating after overnight sample in containing 1 μg/ml meropenem. Forty-three (13.1%) samples were positive by PCR 11 (3.4%) ; none . sensitivity, specificity, predictive value (PPV), negative (NPV) assay 100%, 99.0%, 93.0%, respectively, sequencing target genes. PPV, NPV 99.4%, 81.8%, respectively. Since clinical contained organisms 66 contrived prepared at various dilutions three Klebsiella pneumoniae isolates showed 100% positivity from 300 1,800 CFU/ml 93.3% 150 CFU/ml. Xpert MDRO required 2 min hands-on time 47 complete. Rapid identification colonized multiplex may help hospitals improve infection control activities.