Chondrogenic differentiation of ChM-I gene transfected rat bone marrow-derived mesenchymal stem cells on 3-dimensional poly (L-lactic acid) scaffold for cartilage engineering

摘要: We have explored the role of Chondromodulin-I (ChM-I) in chondrogenesis bone marrow-derived mesenchymal stem cells (BMSCs) 3-dimensional (3D) scaffold for cartilage tissue engineering. BMSCs Sprague Dawley (SD) rats were cultured on poly-(L-lactic acid) [PLLA] scaffolds with different pore sizes (80-200 μm, 200-450 μm) or without surface modification by chitosan. Cell viability, proliferation, and morphology measured using confocal microscope CCK-8 method. Untransfected BMSCs, expressing pcDNA3.1(+), plasmid pcDNA3.1 (+)/ChM-I 3D standard growth medium transforming factor-β1 (TGF-β1) supplemented chondrogenic induction vitro 3 weeks expression collagen type II was determined. Cell-scaffolds constructs implanted subcutaneously months vivo. had a higher viability proliferation PLLA size μm than that 80-200 chitosan did not enhance cell attachment. The ChM-I gene enhanced increased synthesis. Immunohistochemistry from vivo study showed regeneration scaffolds. It also demonstrated TGF-β1 might promote rat synergizing gene. could be beneficial to future applications repair.