Improved Real-Time Scanning Fluorescence Electrophoresis Apparatus for the Analysis of Polynucleotide Fragments
作者： Eric W. Lachenmeier , Louis B. Hoff , Eric S. Nordman , Yefim M. Raysberg
DOI:
关键词: Detector 、 Fluorescence spectroscopy 、 Polynucleotide 、 Electrophoresis 、 Analytical chemistry 、 Chromatography 、 Relative fluorescence units 、 Fluorescence 、 Light source 、 Chemistry
摘要: This invention relates to an improved real-time scanning fluorescence electrophoresis apparatus for the electrophoretic analysis of fluorescently-labeled polynucleotide fragments. The is characterized by having chamber containing separation medium capable accommodating multiple lanes arranged in a planar array, detector mounted on translatable stage, light source exciting fluorescent molecules, and computer collecting data consisting time, location, wavelength intensity information. improvements herein disclosed include: (i) using spectral-array detecting emission from said fragments including simultaneous detection labels, (ii) temperature control means during electrophoresis.
google.nl 参考文章(15)
Michael Schuette, Adjustable-height vertical gel slab electrophoresis apparatus ,(1988)
Lloyd M. Smith, Anthony J. Kostichka, Detection apparatus for electrophoretic gels ,(1991)
William J. Mordan, John D. Lytle, Michael W. Hunkapiller, Charles R. Connell, John A. Bridgham, Real time scanning electrophoresis apparatus for DNA sequencing ,(1986)
Keiichi Nagai, Hideki Kambara, Electrophoretic apparatus having arrayed electrophoresis lanes ,(1991)
Achim E. Karger, Joel M. Harris, Raymond F. Gesteland, Multiwavelength fluorescence detection for DNA sequencing using capillary electrophoresis Nucleic Acids Research. ,vol. 19, pp. 4955- 4962 ,(1991) , 10.1093/NAR/19.18.4955
Janet S. Ziegle, Ying Su, Kevin P. Corcoran, Li Nie, P. Eric Mayrand, Louis B. Hoff, Lincoln J. McBride, Mel N. Kronick, Scott R. Diehl, Application of automated DNA sizing technology for genotyping microsatellite loci Genomics. ,vol. 14, pp. 1026- 1031 ,(1992) , 10.1016/S0888-7543(05)80126-0
Robert L. Brumley, Lloyd M. Smith, Rapid DNA sequencing by horizontal ultrathin gel electrophoresis. Nucleic Acids Research. ,vol. 19, pp. 4121- 4126 ,(1991) , 10.1093/NAR/19.15.4121
Kary Banks Mullis, Process for amplifying nucleic acid sequences ,(1986)
Watkins Pc, Restriction fragment length polymorphism (RFLP): applications in human chromosome mapping and genetic disease research. BioTechniques. ,vol. 6, pp. 310- 322 ,(1988)