Optimization of hydrogen production by the psychrophilic strain G088
作者： Cecilia L. Alvarez-Guzmán , Victor E. Balderas-Hernández , Raúl González-García , José T. Ornelas-Salas , Abraham M. Vidal-Limón
DOI: 10.1016/J.IJHYDENE.2016.08.098
关键词: Yield (chemistry) 、 Polaromonas rhizosphaerae 、 Yeast extract 、 Hydrogen production 、 Psychrophile 、 Tryptone 、 Biochemistry 、 Strain (chemistry) 、 Nuclear chemistry 、 Chemistry 、 Biohydrogen
摘要: Abstract In this study, the response surface method with a Box-Behnken design was applied to evaluate effect of temperature, pH and glucose concentration optimize hydrogen production by psychrophilic strain G088 ([EU636029], which is closely related Polaromonas rhizosphaerae [EF127651]). Biohydrogen performed in 120 cm3 serological bottles medium containing 2.75 g/dm3 tryptone, 0.25 g/dm3 yeast extract range 10–40 g/dm3, 3.0–8.0 temperature 13–37 °C. According mathematical model, 26.30 °C, 6.2 25.31 g/dm3 were optimum conditions for predict (HP) 503.34 cm3, rate (HPR) yield (HY) 37.91 cm3/dm3/h 1.93 mol H2/mol glucose, respectively. The linear quadratic most significant terms affecting HP. Otherwise HPR affected only concentration, while HY three factors. optimal experimentally tested triplicate corroborating correspondence predicted value model. experimental values production, under these 513 ± 12.48 cm3, 36.5 ± 4.10 cm3/dm3/h 1.81 ± 0.04 mol
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