Development of a real-time polymerase chain reaction assay for the diagnosis of human herpesvirus-6 infection and application to bone marrow transplant patients
作者： Agnès Gautheret-Dejean , Chaysavanh Manichanh , Florence Thien-Ah-Koon , Anne-Marie Fillet , Nicole Mangeney
DOI: 10.1016/S0166-0934(01)00390-1
关键词: Herpesviridae 、 Virology 、 Peripheral blood mononuclear cell 、 Real-time polymerase chain reaction 、 Biology 、 Bone marrow 、 Viral load 、 Human herpesvirus 6 、 Amplicon 、 Polymerase chain reaction
摘要: A quantitative real-time PCR assay was developed for human herpesvirus-6 (HHV-6) genome based on TaqMan® technology. After choosing a region of interest into the U65–U66 genes of HHV-6 genome, its nucleotide sequence was determined among four HHV-6 strains (one variant A and three variants B) to exclude a variability of sensitivity due to interstrain sequence differences. A plasmid containing HHV-6 target sequences identical to those of reference type viruses was constructed with the aim of standardisation. This HHV-6 …
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